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Effects of Ischemic Preconditioning on Injury of Rat Skeletal Muscle During Use of Tourniquet
https://doi.org/10.57457/00000012
https://doi.org/10.57457/000000126dee9655-e79e-4245-9bc8-0c3adc5722fb
| 名前 / ファイル | ライセンス | アクション |
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本文 (2.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2022-09-27 | |||||||||
| タイトル | ||||||||||
| タイトル | Effects of Ischemic Preconditioning on Injury of Rat Skeletal Muscle During Use of Tourniquet | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | preconditioning | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | ischemic reperfusion injury | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | skeletal muscle | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | apoptosis | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.57457/00000012 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| 著者 |
森川 高宗
× 森川 高宗
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| 著者（英） | ||||||||||
| 姓名 | Takanori Morikawa | |||||||||
| 言語 | en | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | [Purpose] The use of a pneumatic tourniquet for an extended period of time in limb surgery can lead to apoptosis after reperfusion. While ischemic preconditioning (IPC) has a protective effect on reperfusion injury, the effect of age is unknown. This study thus examines the effects of IPC and age on reperfusion injury in the skeletal muscle. [Methods] A pneumatic tourniquet was applied on the hindlimb of rats aged 3–24 months with a tourniquet pressure of 300 mmHg. The IPC⊖ group had two 120-minute tourniquet applications with a 5-minute reperfusion period in between, followed by reperfusion. The IPC⊕ group underwent 5 minutes of tourniquet application and 5 minutes of reperfusion repeated 3 times. The gastrocnemius muscles were then excised 72 hours after reperfusion. The ratio of apoptotic cells was compared using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The expression of apoptosis inducers was examined by Western blot and immunostaining. Six-month-old rats were treated as in the experiment described above, and IPC⊖ and IPC⊕ groups were prepared. Gastrocnemius muscles were then harvested immediately after reperfusion and 24 hours and 72 hours after reperfusion. The temporal changes in VEGF and 8-OHdG expression levels were examined by immunostaining. [Results] Degenerated and atrophied myocytes were observed in the gastrocnemius muscles of 3-month-old to 12-month-old rats, whereas partial necrosis and inflammatory cell infiltration were observed in those of 24-month-old rats. Compared with the IPC⊖ group, the ratio of apoptotic cells decreased in the 6- and 12-month-old IPC⊕ rats. Immunostaining and Western blotting showed that Bax expression decreased in the 6-month-old IPC⊕ rats, whereas Bax and caspase-9 expression decreased in the 12-month-old IPC⊕ rats. [Conclusions] IPC⊕ suppresses apoptosis after the application of pneumatic tourniquet. However, apoptosis cannot be prevented when IPC⊕ is applied in elderly groups. Age-related changes in myocyte mitochondria may be involved in the apoptotic and necrotic process due to ischemia-reperfusion injury in elderly patients despite IPC. |
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| 書誌情報 |
金沢医科大学雑誌 en : Journal of Kanazawa Medical University 巻 46, 号 2, p. 42-50, 発行日 2021-09 |
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| 出版者 | ||||||||||
| 出版者 | 金沢医科大学医学会 | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 0385-5759 | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
